Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 21;2:16020. doi: 10.1038/cddiscovery.2016.20

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

APRIL is present in the CLL LN and CLL cells express APRIL receptors. (a) After total RNA lysis of paraffin-embedded LN material or control NIH-3T3 (3T3) cells, APRIL mRNA levels were determined by performing a qPCR on CLL LN material (N=3) and an SLE LN as positive and 3T3 cells as negative control. All qPCRs were performed in triplo. a.u. denotes arbitrary units. (b) Paraffin embedded LN slides from six CLL patients were immunohistochemically stained for APRIL, macrophage marker CD68, proliferation marker Ki67, and nuclear counterstain methyl green (MG). Data shown are representative of N=6. Scale bar represents 200 μm (left) or 50 μm (right). (c) CLL cells (N=6) isolated from PB were stained for APRIL receptors TACI and BCMA or with the relevant isotype controls and analyzed by flow cytometry. Bars show mean±S.E.M. **P<0.01 in a paired t-test.