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BTCI-induced mitochondrial membrane depolarization. (a) MCF-7 and (b) MDA-MB-231 cells were incubated with BTCI at the concentration of 150 μM and 100 μM, respectively, for 24 h. Dye-loading solutions were added to cells and incubated for 30 min. The change of mitochondrial membrane potential was measured by using Mitochondrial Membrane Potential Kit (Sigma). Results are presented as mean±S.D. of two separate experiments conducted in triplicate, *P<0.01 versus untreated cells.
