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. 2016 Mar 21;2:15067. doi: 10.1038/cddiscovery.2015.67

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Copyright © 2016 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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mRNA expression of IL-8, NFKB2, CFLAR, BAX and BCL-2 quantified by RT-PCR in human breast adenocarcinoma cells. Cells were treated with BTCI for 24 h, and RNA was extracted. This RNA was used to prepare cDNA that was subject to real-time PCR to quantify the mRNA level of each gene. Each sample was run in triplicate, and the relative amount of mRNA was normalized to the β-actin content in each sample. Normalized expression values (specific gene expression levels/β-actin expression levels) are presented. Data points are represented by the expression ratio and mean±S.D. fold of control in MCF-7 (a and b) and MDA.MB.231 (c and d). Each RNA sample represents a pool of RNA samples from two independent biological experiments conducted in triplicate, *P<0.05 versus untreated cells.