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. 2016 Mar 21;2:16011. doi: 10.1038/cddiscovery.2016.11

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Copyright © 2016 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Distinct kinetic patterns between the pan-nuclear responses and the repair foci of γH2AX, p-ATM and p-DNA-PKcs. (a) Representative images of X-irradiation-induced pan-nuclear signals and foci of γH2AX, p-ATM and p-DNA-PKcs in resting HPBLs at 6 h after 0.5 Gy X-ray exposure. ×60 magnification, ×1.0 zoom. (b) HPBLs were irradiated with 0.5 Gy X-ray, and the pan-nuclear signals and foci of γH2AX (left panel), p-ATM (middle panel) and p-DNA-PKcs (right panel) were examined at the indicated time points after irradiation and quantified by counting 1000–5000 HPBLs from each sample. γH2AX, p-ATM and p-DNA-PKcs were labeled in red, and the nuclei were stained in blue with DAPI. Data are presented as the means±S.D. of four to six donors.