Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 21;2:16011. doi: 10.1038/cddiscovery.2016.11

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

KU55933, PFT-μ and ZVAD-fmk inhibited the proliferative response to mitogen in resting HPBLs exposed to X-ray irradiation. HPBLs were treated with 10 μM KU55933 for 2 h (a), 20 μM PFT-μ for 5 h (b) or 100 μM ZVAD-fmk for 1 h (c) followed by irradiation with 5 Gy X-ray, and were cultured with PHA and colcemid for 48 h. HPBLs were then collected and transformed HPBLs were detected using the Ki67 antibody. A total of 1000 HPBLs from each sample were examined to determine the proliferation ratio. Data are presented as the means±S.D. obtained from three donors. *P<0.05 and **P<0.01 compared with corresponding non-irradiated HPBLs with/without inhibitor treatment; ^§ P<0.05 and ^§§ P<0.01 compared with corresponding HPBLs without inhibitor treatment. (d) Representative images of HPBLs labeled with Ki67. The cells were treated with KU55933 and irradiated with X-ray as described above. Ki67 was labeled in red and nuclei were stained in blue with DAPI. ×40 magnification, ×1.0 zoom.