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. 2015 Sep 28;1:15037. doi: 10.1038/cddiscovery.2015.37

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Copyright © 2015 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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DIDS inhibits STS-induced caspase activation in HeLa cells. Cells that were preincubated with or without DIDS (50 or 500 μM) for 30 min were incubated with STS 1 μM for 4 h and lysed to determine caspase activity, which was normalized based on the response obtained with STS. Panels show caspase-3 (a, n=7), capase-9 (b, n=4) and caspase-8 (c, n=4) activities without (total activity, solid columns) and with caspase inhibitors (open columns), which were Ac-DEVD-CHO for caspase-3 (a), Ac-LEHD-CHO for caspase-9 (b) and Ac-IETD-CHO for capase-8 (c).ln all cases, STS induced a significant caspase activity with respect to control (**P<0.05). *^,&,#P<0.05, Tukey multiple comparison test. *DIDS 500 μM increased DEVDase activity unrelated to caspase-3 when compared with control activity. ^&STS-induced caspase-3 activity is significantly smaller in the presence of either 50 or 500 μM DIDS. ^#There is a small but significant caspase-3 activity resistant to DIDS.