Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 28;1:15037. doi: 10.1038/cddiscovery.2015.37

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

DIDS inhibits caspase-3 processing, PARP degradation and apoptotic nuclei features. HeLa cells preincubated with or without DIDS (50 or 500 μM) for 30 min before incubation with STS (1 μM) for 4 h were lysed and (a) pro-caspase 3 was detected with antibody (clone 3G2, n=3) and (b) PARP fragmentation with antibody (clone 4C10-5, n=5). DIDS totally abolished procaspase-3 proteolytic processing and strongly diminished STS-induced PARP fragmentation. (c) Apoptotic nuclei were induced by STS (4 h incubation). However, neither the vehicle (DMSO, 0.1 or 1%) nor DIDS alone induced apoptotic nuclei. Preincubation with DIDS (50 μM) strongly inhibited STS-induced apoptotic nuclei features. Interestingly, this inhibitory effect was a little bit smaller for a 10 times higher concentration of DIDS (500 μM) *^,&P<0.05, Tukey multiple comparison test. *respect the control, ^&respect the effect of STS.