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. 2016 May 9;2:16010. doi: 10.1038/cddiscovery.2016.10

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Copyright © 2016 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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OR51B5 is expressed in the CML cell line K562. (a) RT-PCR experiments show the expression of OR51B5 in K562 cells at the expected size of ~230 bp. GAPDH was used as a control. (b) qPCR experiments showed that after 4 h isononyl alcohol incubation OR51B5 expression was significantly decreased. The effect was enhanced by longer stimulation time (24 h). (c) Immunocytochemical staining with the OR51B5 antibody validates the expression of OR51B5 in K562 cells. DAPI was used to stain the cell nuclei.