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. 2016 May 9;2:16027. doi: 10.1038/cddiscovery.2016.27

Figure 5.

Figure 5

Autophagy deficiency was associated with the sensitivity of CRC cells to GW8510. (a) The protein expressions in HCT116, LoVo, HCT15, DLD-1, and HT-29 cells were analyzed by western blots. The ratio of LC3-II to LC3-I or GAPDH was quantified. (b) HCT116 cells were treated the different doses of GW8510 for 72 h in the absence or presence of 10 nM bafilomycin. The cell viability was determined by an MTT assay. (c) HCT116 cells were treated with different doses of rapamycin (Rapa) for 24 h. The protein expressions were analyzed by western blots. (d) ATG7-wild-type (ATG7-WT) and ATG7-knockout (ATG7-KO) DLD-1 cells were treated with different doses of GW8510 for 72 h. The cell viability was determined by an MTT assay.