Table 1. Effect of Imeglimin on respiratory chain complex I activity in HMEC-1 cells.

	Control	Imeglimin 100 μM – 24 h	Imeglimin 10 mM – 4 h
Total activity J NADH (nmol NADH/min/107 cells)	1.43±0.034	1.44±0.042	1.39±0.049
After addition of rotenone (nmol NADH/min/107 cells)	0.39±0.035	0.38±0.049	0.39±0.043
Rotenone-sensitive Complex I activity (nmol NADH/min/107cells)	1.035±0.027	1.056±0.022	0.99±0.033

HMEC-1 cells incubated in the absence or presence of either 100 μM Imeglimin for 24 h or 10 mM Imeglimin for 4 h were first permeabilized and then submitted to a hypotonic shock as described in the Material and Methods section. NADH oxidation rate was measured fluorimetrically in the presence of decylubiquinone before and after addition of rotenone. Results are means±S.E.M. of four independent experiments.