Table 2. Comparative effects of Imeglimin and Metformin on cellular oxygen consumption (JO2) and energy metabolism.
| Control | Imeglimin | Metformin | |
|---|---|---|---|
|
JO2 (Natom O/min/mg protein) | |||
| No addition | 4.9±0.14 | 4.6±0.22 | 3.1±0.14a |
| + Oligomycin | 1.6±0.04 | 1.5±0.03 | 1.3±0.07 |
| + DNP | 9.1±0.76 | 9.2±0.41 | 6.1±0.51a |
| J lactate (μmol NADH/30 min/mg protein) | 3.65±0.27 | 3.81±0.30 | 4.90±0.29a |
| Lactate/pyruvate ratio | 1.59±0.11 | 1.65±0.13 | 2.23±0.12a |
| ATP (nmol/mg prot) | 83.9±4.37 | 81.6±1.7 | 69.7±4.95a |
| ADP (nmol/mg prot) | 17.28±3.07 | 17.38±1.38 | 21.02±2.78 |
| ATP/ADP | 5.09±0.70 | 4.8±0.25 | 3.37±0.23a |
HMEC-1 cells incubated in the presence of either 10 mM Imeglimin for 4 h or 10 mM Metformin for 30 min. JO2 was measured before and after the successive additions of oligomycin and DNP. In parallel experiment, the production of lactate and the lactate-to-pyruvate ratio, as well as the content of ATP and ADP, was determined as described in the Material and Methods section. Results are means±S.E.M. of four independent experiments.
a
P<0.05 versus control cells, paired Student’s t -test.