Table 3. Effect of Imeglimin on mitochondrial H₂O₂ production.

	H2O2 (pmol/min/107 cells)
	Glutamate/malate		Succinate		Glutamate/malate+succinate
	Control	Imeglimin	Control	Imeglimin	Control	Imeglimin
Basal	32.3±2.4	28.4±2.2	25.7±3.0	14.5±1.3a	22.3±1.5	15.7±1.4a
+Rotenone	38.3±3.2	37.5±1.5	10.7±1.8	11.4±2.7	7.8±0.9	6.9±1.1
+Antimycin A	51.0±4.8	51.4±8.7	20.1±3.3	19.1±3.2	20.5±1.4	18.6±1.3

HMEC-1 cells incubated in the absence or presence of 100 μM Imeglimin for 24 h were digitonin-permeabilized in a KCl respiratory medium supplemented with Amplex Red/HRP. Mitochondria were energized with glutamate/malate, succinate or both substrates (glutamate/malate plus succinate) in order to measure the basal H₂O₂ production, then 5 μM rotenone and 0.25 μM antimycin A were added. Results are mean±S.E.M. of five different experiments.

^aP<0.05 versus control, paired Student’s t-test.