Figure 3.

Evolutionary relationships of taxa with DD-containing TNFRs. The evolutionary history was inferred using the neighbor-Joining method.⁴⁴ The optimal tree with the sum of branch length=30.30949398 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown above the branches.⁵² The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method⁵³ and are in the units of the number of amino acid substitutions per site. The analysis involved 62 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 102 positions in the final dataset. Evolutionary analyses were conducted in MEGA7.^54,55 Abbreviations of taxa include: Homo sapiens (Hos), Mus musculus (Mum), Danio rerio (Dar), Xenopus laevis (Xel), Branchiostoma floridae (Brf), Strongylocentrotus purpuratus (Stp), Crassostrea gigas (Crg), Chlamys farreri (Chf), Nematostella vectensis (Nev), and Acropora digitifera (Acd) with a black circle next to taxon name. For full description of sequences, see Supplementary Table 1.