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. 2015 Nov 9;1:15046. doi: 10.1038/cddiscovery.2015.46

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Copyright © 2015 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Gingival fibroblast was treated with LPS (1 μg/ml) for 24 h. Reactive oxygen species (ROS) were determined by DCFH-DA staining. (a) LPS increases ROS accumulation in gingival fibroblast (magnification ×100). (b) The ROS-positive cells were counted by flow cytometry. The experiment was repeated three times with similar results (Ctrl, control; **P<0.01).