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. 2015 Nov 9;1:15046. doi: 10.1038/cddiscovery.2015.46

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Copyright © 2015 Cell Death Differentiation Association

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DNA damage appeared after stimulation of LPS for 48 h. Immunofluorescent localization of γH2AX in gingival fibroblasts was performed following 1 μg/ml LPS treatment for 6, 24 and 48 h. (a and b) γ-H2AX was not detected in 6 and 24 h, respectively. (c) But γ-H2AX was increased in cell nuclei by LPS in 48 h (red arrow; Ctrl, control; magnification ×400). (d) LPS (1 μg/ml) increased p53 and p21 in 24 h.