Figure 4. Eμ:3′Eα Complex Formation and Recruitment of GLT Promoters Is Not Dependent on the cEμ Enhancer Element.

Relative crosslinking frequencies for two independent chromatin template preparations are shown for CD43− resting splenic B cells (R) from WT and cEμ Δ/Δ mice (129Sve strain) that were unstimulated or activated with LPS alone or LPS+IL4 for 48 hr. Eμ and 3′Eα hs1,2 were used as anchor fragments (A and G) that were used along with fragments from the rest of the locus, as indicated. Data represent standard errors of the mean values. Student’s t test was used to determine the significance of the different crosslinking frequencies in chromatin samples (*p < 0.05; **p < 0.001).