Figure 7.
CS exposure effects on Akt pathways. Liver protein homogenates were used in bead-based multiplex ELISAs to measure immunoreactivity to: A) Akt, B) GSK-3β, C) PRAS40, D) p70S6K, E) pS473 AKT, F) pS9-GSK-3β, G) pT246-PRAS-40, and H) pTpS421/424-p70S6K. Relative phosphorylation is represented by the calculated ratios of: I) pS473-/total AKT, J) pS9-/total GSK-3β, K) pT246-/total PRAS40, and L) pTpS421/424-/total p70S6K. Data were analyzed by 1-way ANOVA (Table 1). Post-hoc Tukey multiple comparison test results are depicted in the graphs (*P<0.05; **P<0.01; ***P<0.001; ****P<0.0001).