Table 1.
PCR Primer Pairs and Probes used for Duplex qRT-PCR Analysis.
| mRNA | UPL primers | Sequence 5′-3′ | Amplicon | Position | Probe ID# |
|---|---|---|---|---|---|
| mINS-UPL-For | GGCATTGTGGATCAGTGCT | 75bp | NM_008386.3 | 32 | |
| Insulin | mINS-UPL-Rev | AGGTGGGCCTTAGTTGCAG | 445-519 | ||
| mINR-UPL-For | GCCAAGTAGCATCATGTCCA | 73bp | NM_010568.2 | 52 | |
| Insulin Receptor | mINR-UPL-Rev | ACATGCAGAACAATTTCAGTCC | 9225-9297 | ||
| mIGF1-UPL-For | ACATAGGTGGCCTTCTCCAG | 60bp | NM_010512.4 | 4 | |
| IGF-1 | mIGF1-UPL-Rev | TGGAATCACCAAATCCAACC | 3411-3470 | ||
| mIGF1R-UPL-For | GTGCTCTGCCTCTGTCCAC | 65bp | NM_010513.2 | 84 | |
| IGF-1 Receptor | mIGF1R-UPL-Rev | TGGAGTTGAGGATTGCACTG | 8234-8298 | ||
| mIGF2-UPL-For | CGTCCCTCAGTGTCATCTTG | 69bp | NM_010514.3 | 73 | |
| IGF-2 | mIGF2-UPL-Rev | TGTAGAGCTCCAGACCTCCTG | 3096-3164 | ||
| mIGF2R-UPL-For | CCTTCTCTAGTGGATTGTCAAGTG | 62bp | NM_010515.2 | 33 | |
| IGF-2 Receptor | mIGF2R-UPL-Rev | AGGGCGCTCAAGTCATACTC | 3387-3448 | ||
| Insulin Receptor Substrate, Type 1 | mIRS1-UPL-For mIRS1-UPL-Rev | GAGGAGCAACACTCTACTTCTGG AACCCTTGCTCCTTCCTTG |
70bp |
NM_010570.4 6275-6344 |
79 |
| Insulin Receptor Substrate, Type 2 | mIRS2-UPL-For mIRS2-UPL-Rev | GGTTTCATGTCCCTTGACGA CTGTGGCTACTGAAGGCTCTC |
65bp |
NM_001081212.1 1477-1541 |
19 |
| Insulin Receptor | mIRS4-UPL-For | GTCTGCAAACGCGGCTAC | 61bp | NM_010572.2 | 38 |