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. Author manuscript; available in PMC: 2016 Aug 10.
Published in final edited form as: Biochem J. 2011 May 1;435(3):651–660. doi: 10.1042/BJ20101810

Figure 6.

Figure 6

Oligomeric state of REJ domain 2. A: Sedimentation equilibrium AUC results at 18 000 rpm (upper trace) and 25 000 rpm (lower trace) of refolded protein in NV10. For clarity only one loading concentration has been shown. The determined molecular weight was 15.2 KDa, close to the expected monomeric weight of 15.7 KDa. Fits were determined globally using 6 data sets using the program SEDPHAT [26]. B: Analytical gel filtration of domain 2 expressed in the soluble showing one peak at 12.6 mL which corresponds to a molecular weight of 15 kD.