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1D NMR spectra of domains 1 and 2. A: 1D 1H spectrum of refolded domain 1. B: 1D 1H spectrum of refolded domain 2. C: 1D 1H spectrum of soluble expressed domain 2. For all three only the low- and highfield portions of the spectra are shown. Spectra were recorded with samples at a concentration of 100 μM at 800 MHz in measurement buffer at 25 °C.
