Table 1.

FNIII domain constructs used in this work as shown in Fig. 3A and the results of their bacterial expression. Note that for constructs that did not express as soluble protein the protein yield refers to soluble protein obtained after Nvoy assisted refolding. Column headings: D: Domain; Start/End: position of first and last residue in full length human polycystin 1; Size: number of amino acids of polycystin 1 in the construct; MW: molecular weight in kD; ε: molar extinction coefficient in M⁻¹ cm⁻¹; Host: bacterial expression host ArcticExpress (AE) or BL21* (Star); Soluble: is the protein expressed soluble?; Yield: amount of purified, soluble protein in mg from 1L LB culture; Solubility: rough qualitative estimate of protein solubility after purification.

D	Start	End	Size	MW	ε	Host	Soluble	Yield	Solubility	Comments
D1	2152	2262	111	12.6	12950	Star	NO	50	Modest	-
D1	2152	2262	111	12.6	12950	AE	YES	1	Modest	soluble aggregates
D1	2155	2254	100	11.4	12950	Star	NO	15	Poor	-
D1	2155	2254	100	11.4	12950	AE	NO	-	-	-
D2	2257	2369	113	12.3	20970	Star	NO	50	Good	-
D2	2257	2369	113	12.3	20970	AE	YES	2	Good	-
D2	2257	2374	118	12.9	20970	Star	NO	45	Modest	-
D2	2257	2374	118	12.9	20970	AE	YES	1	Modest	precipitated after short time
D2	2282	2361	80	8.8	13980	Star	NO	~2	Very poor	-
D2	2282	2361	80	8.8	13980	AE	NO	-	-	-
D3	2371	2474	104	11.2	11460	Star	NO	20	Modest	degradation
D3	2374	2474	101	10.9	11460	Star	NO	30	Modest	degradation
D3	2374	2474	101	10.9	11460	AE	NO	-	-	-
D3	2392	2463	76	8.2	6990	Star	NO	10	Poor	degradation
D4	2468	2577	110	11.8	9970	Star	NO	-	-	Very poor expression
D4	2477	2577	101	10.9	9970	Star	NO	-	-	Very poor expression
D4	2477	2577	101	10.9	9970	AE	NO	-	-	Very poor expression
D4	2485	2573	93	10	9970	Star	NO	-	-	Very poor expression