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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Circ Heart Fail. 2016 Aug;9(8):10.1161/CIRCHEARTFAILURE.116.003129 e003129. doi: 10.1161/CIRCHEARTFAILURE.116.003129

Figure 4. Inducible cardiac-specific deletion of Kindlin-2 in adult cardiomyocytes mimics early loss of Kindlin-2 in the heart.

Figure 4

(A) Western blot and (B) corresponding quantitative densitometric analysis of Kindlin-2 (KN2) and integrin β1D in the heart of KN2f/f αMHC-MerCreMer mice 3 months after injection with tamoxifen (KN2f/f Cre). KN2f/f mice injected with tamoxifen, and KN2f/f and KN2f/f Cre mice injected with oil served as littermate controls. GAPDH served as a loading control. Kindlin-2 and integrin β1D pixel density is normalized to the level of GAPDH (*P<0.05, two-tailed Student’s t-test). A representative example of at least two independent experiments is shown (n=2 per genotype/experiment). (C) Kaplan-Meier survival curves of KN2f/f and KN2f/f Cre mice injected with oil or tamoxifen (Tam) show that Tam-treated KN2f/f Cre mice die between 40–56 weeks. (D) Macroscopic cross-sectional views of Hematoxylin and Eosin- and Masson’s Trichrome-stained hearts isolated from KN2f/f and KN2f/f Cre mice, 13 months following injection with oil or tamoxifen (scale bar: 2 mm). Representative high magnification views are shown below each corresponding whole heart section (scale bar: 100 µm) (n=2–3 per genotype). (E) Echocardiographic analysis of fractional shortening (FS); left ventricle internal diameter, diastole (LVIDd); LVID, systole (LVIDs); interventricular septum diameter (IVSd); and LV posterior wall, diastole (LVPWd), in KN2f/f and KN2f/f Cre mice, 4, 7, 11, and 13 months following injection with oil or tamoxifen (**P<0.01, ***P<0.001, ****P<0.0001, two-way ANOVA (Tukey post-hoc analysis); n=noted in each bar).