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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Mol Pharm. 2016 Jun 27;13(8):2605–2621. doi: 10.1021/acs.molpharmaceut.5b00948

Figure 9.

Figure 9

Confocal microscopy images of cellular interaction of different complexes with the HCC1954 cell line after 4 h incubation with siRNA polyplexes. HCC1954 cells were seeded with 25,000 cells per chamber and transfected with 25 pmol of siRNA (TYE 563 labeled, indicated by red color) for 4 h. Cells were fixed and the nuclei stained with DAPI (indicated by blue color). Representative pictures are shown (100× magnification): (A) blank; siRNA polyplexes with (B) Lipofectamine; (C) Tf–PEI (N/P 7); and (D) PEI (N/P 7).