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. 2016 Jul 11;12:1428–1433. doi: 10.3762/bjoc.12.137

Figure 2.

Figure 2

a) Inhibitory effect of compound 4 (200 μM suspension in DMSO) on pyocyanin production in PAO1. DMSO was added as a control. No effect on growth was observed for the compound. b) Effect of various concentrations of 4 on pyocyanin production in PAO1. A logarithmic trend line has been fitted to the averaged data points (calculated using Microsoft® Excel® 2011). Growth conditions in both cases: cultures of PAO1 were grown in Luria broth medium in the presence of compound 4 (at the indicated concentration) with good aeration at 37 °C for 13 hours (initial OD600 of 0.05 t = 0). After growth, pyocyanin production was quantified as previously described [29]. The data represents the averages and standard deviations from the results of three independent biological repeats.