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The target duplex contains a 29 base pair fragment from HIV proviral DNA [35] and a T4 hairpin is connecting two strands: a 16 base pair polypurine-polypyrimidine tract (TFO-binding site – is indicated by a red rectangle), an adjacent overlapping poly(dA:dT) tract (MGB-binding site – by a blue rectangle). Fluo is a fluorescein label.
