Table 2. Effect of butyrate glycerides on concentrations of serum metabolites as well as total protein and selected key enzymes in selected tissues in broilers.
| Tissue | Item | BD-fed group | BG-fed group | P-value | Pooled SEM |
|---|---|---|---|---|---|
| Serum | Glucose (mmol/L) | 14.0 | 13.3 | 0.10 | 0.28 |
| Total cholesterol (mmol/L) | 4.0 | 3.6 | 0.04 | 0.09 | |
| Triglycerides (mmol/L) | 1.21 | 1.0 | 0.04 | 0.05 | |
| HDL-C (mmol/L) | 2.9 | 2.6 | 0.03 | 0.08 | |
| LDL-C (mmol/L) | 0.9 | 0.8 | 0.07 | 0.08 | |
| Total protein (g/L) | 18.9 | 22.8 | 0.00 | 0.24 | |
| ACACB (mg/L) | 65.2 | 47.3 | 0.01 | 1.88 | |
| FASN (mg/L) | 5.1 | 2.3 | 0.00 | 0.19 | |
| LPL (ng/L) | 93.8 | 88.4 | 0.08 | 0.87 | |
| Jejunum | Total protein (mg.g-1 tissue) | 227.1 | 281.1 | 0.00 | 0.36 |
| ACACB (μg.g-1 protein) | 49.1 | 40.7 | 0.06 | 0.31 | |
| FASN (μg.g-1 protein) | 1.5 | 2.0 | 0.07 | 0.59 | |
| LPL (μg.g-1 protein) | 106.4 | 88.6 | 0.01 | 0.08 | |
| Liver | Total protein (mg.g-1 tissue) | 352.1 | 311.8 | 0.09 | 0.24 |
| ACACB (μg.g-1 protein) | 13.5 | 8.5 | 0.00 | 1.82 | |
| FASN (μg.g-1 protein) | 37.4 | 28.4 | 0.00 | 0.09 | |
| LPL (μg.g-1 protein) | 49.5 | 39. | 0.02 | 0.77 | |
| Abdominal adipose | Total protein (mg.g-1 tissue) | 27.8 | 33.8 | 0.14 | 1.88 |
| ACACB (μg.g-1 protein) | 2.9 | 2.1 | 0.06 | 0.19 | |
| FASN (μg.g-1 protein) | 4.2 | 1.7 | 0.00 | 0.57 | |
| LPL (μg.g-1 protein) | 10.4 | 7.1 | 0.02 | 0.86 |
Notes: agents and kit information for the assays. Total protein (Cat #: 22660, Thermo Scientific Int., Dockford, IL, USA), ACACB (Cat #: E12A0639, Shanghai Blue-Gene Biotech Co., Ltd., Shanghai, China), FASN (Cat #: SEC470Ga, Uscn Life Science Inc., Wuhan, Hubei, China); LPL (Cat #: CSB-E13395C, Cusabio Biotech Co., Ltd., Wuhan, Hubei, China). n = 6 birds. ACACB: acetyl coenzyme A carboxylase β; BD: basal diet; BG: butyrate glycerides; FASN: fatty acid synthase; HDL-C: high-density lipoprotein-cholesterol; LDL-C: low-density lipoprotein-cholesterol; LPL: lipoprotein lipase.