Table 7. Comparison in the results of RNA-seq and qPCR analyses of selected genes involved in PPAR-α signalling pathway in respond to butyrate glycerides treatment in broilersa.
| Tissue | Gene | RNA-seq | qPCR | ||
|---|---|---|---|---|---|
| Log2 fold-change | P value | Log2 fold-change | P value | ||
| Jejunum | FABP-4 | -1.55 | < 0.001 | -1.12 | < 0.01 |
| LPL | -1.04 | < 0.001 | -1.00 | < 0.001 | |
| MMP-1 | 1.85 | < 0.001 | 1.65 | < 0.001 | |
| PLIN-1 | -1.58 | < 0.001 | -0.62 | < 0.01 | |
| Liver | APOA5 | -1.19 | < 0.001 | ND | ND |
| CYP8B1 | -1.64 | < 0.001 | -1.51 | < 0.001 | |
| FABP-2 | -2.93 | < 0.001 | -1.89 | < 0.001 | |
| LPL | -1.08 | < 0.001 | -1.25 | < 0.001 | |
| MMP-1 | 2.41 | < 0.001 | 0.78 | 0.07 | |
β –actin: actin, beta (HGNC: 132); APOA5: apolipoprotein A-V (HGNC: 17288); CYP8B1: cytochrome P450, family 8, subfamily B, polypeptide 1 (HGNC: 2653); FABP2: fatty acid binding protein 2, intestinal (HGNC: 3556); FABP4: fatty acid binding protein 4, adipocyte (HGNC: 3559); LPL: lipoprotein lipase (HGNC: 6677); MMP1: matrix metallopeptidase 1 (HGNC: 7155); PLIN1: perilipin 1 (HGNC:9076).
a n = 6 chickens;
ND: not determined.