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. 2016 Aug 10;11(8):e0160860. doi: 10.1371/journal.pone.0160860

Fig 3. Hypo-phosphorylated IκBβ at S313 improves survival and decreases inflammatory cytokines in CLP sepsis.

Fig 3

(A)The expression of EGFP in Tg mice post CLP. mRNA level of EGFP was examined by real-time PCR (n = 3). (B) Tg mice overexpressing hypo-phosphorylated IκBβ at Ser-313 and WT mice were age- and sex-matched before performing the CLP operation(n = 20). The survival rates were observed and compared out to 72 hours by log-rank test (p< 0.01). (C-D)Serum IL-6 and TNF-α levels (ng/mL) at different time-points afterCLP were detected by ELISA (n = 6).(E-F) mRNA levels of IL-6 and TNF-αwere examined by real-time PCR (n = 6). Figures are representative of three independent experiments. Values were presented as mean ± SEM,*p< 0.05, **p< 0.01,one-way analysis of variance (ANOVA) and Bonferroni’s multiple comparison tests, log-rank test.