| Parameter | Without clusterin (n) | With clusterin (n) |
|---|---|---|
| Rate of monomer and oligomer release (s−1) | (9.3 ± 3.1)×10−5 (12) | (1.7 ± 0.3)×10−5 (8) |
| Rate of clusterin release (s−1) | — | (9.8 ± 0.9)×10−7 (3) |
| Final concentration of all species (nM) | 270 ± 20 (12) | 120 ± 20 (8) |
| Final concentration of oligomeric species (nM) | 0.16 ± 0.06 (12) | 0.42 ± 0.1 (8) |
| Final soluble clusterin concentration (nM) | — | 90 ± 14 (3) |
| Aggregation | ||
| ΔG° (dimer) (kJ mol−1) | −23.0 ± 2.9 (4) | — |
| ΔG° (larger oligomer) (kJ mol−1) | −39.3 ± 3.0 (11) | — |
| Disaggregation | ||
| ΔG° (dimer) (kJ mol−1) | −18.2 ± 0.5 (3) | −25.8 ± 2.6 (4) |
| ΔG° (larger oligomer) (kJ mol−1) | −38.9 ± 2.7 (12) | −43.9 ± 1.0 (12) |
| ΔH° (dimer) (kJ mol−1) | −43.8 ± 24.5 (3) | — |
| ΔH° (larger oligomer) (kJ mol−1) | −29.1 ± 0.3 (12) | — |
| ΔS° dimer (kJ mol−1) | −80.8 ± 83.5 (3) | — |
| ΔS° larger than dimer (kJ mol−1) | 28.3 ± 1.1 (12) | — |
The rates were derived from fitting monomolecular dissociation functions to the plots of species released as a function of time from disaggregation experiments. All thermodynamic values—free energies (ΔG°), enthalpies (ΔH°), and entropies (ΔS°) of formation for various sizes of oligomers—were determined from the steady state apparent size distributions of the various species. Errors for rate values are s.e.m. and errors for thermodynamic values are s.d.