Extended Data Figure 1.

Pharmacological analyses of A_2AR–mini-Gs complexes. Competition assays were performed on A_2AR expressed in HEK293 cell membranes with the agonist NECA competing for the binding of radiolabelled inverse agonist ³H-ZM241385. Experiments were performed in the presence of either 100 mM KCl (a,b), 100 mM NaCl (c, d) or 500 mM NaCl (e, f) to confirm the similar behaviour of mini-Gs with heterotrimeric G_s with nanobody Nb35 for stabilisation of the complex. Results are summarised in the Table (g). Data from at least 3 independent experiments performed in duplicate were analysed with an unpaired t-test for statistical significance.