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. 2016 Aug 10;10(8):e0004848. doi: 10.1371/journal.pntd.0004848

Fig 3. Activation of p38/JNK (A) and p-IκBα (B) in peritoneal murine macrophages (C57BL/6, TLR2 -/- and TLR4 -/-) by L. amazonensis LPGs (PH8 and Josefa).

Fig 3

Macrophages were stimulated for 5, 15, 30, 45 and 60 min with 10 μg/mL of LPG from L. amazonensis PH8 and Josefa strains. Dually phosphorylated MAPKs (p38 and JNK) and p-IκBα were detected by Western blot analysis. C- = negative control; C+ = E. coli extract, positive control (100 ng/mL, 45 min). Total p38 content was used as the normalizing protein.