Fig 4. LPGs purified of L. amazonensis do not induce translocation of NF-κB through TLRs.
CHO cells expressing TLR2 (TLR2+), TLR4 (TLR4+), or neither (TLR2-/TLR4-) were either untreated (purple line) or treated (green line) with LPGs from both strains of L. amazonensis. Legend: PH8 and Josefa LPGs (0.2 and 0.02 μg), Controls: LPS (TLR4 control) and S. aureus (S.a.) (TLR2 control). CD25 expression was measured by flow cytometry 18 h after stimulation. Results shown as percentage of CD25 expression on stimulated cells minus percentage of CD25 expression on non-stimulated cells.