Table 1.

Primer sequences and PCR conditions for APOBEC3 mRNA

Primers	Primer DNA sequences	PCR conditions
NPM-A3A-F	5'-CAGGAACCGAGAAGAGACAAGCACAT-3'	94 ℃ 5 min, (94 ℃ 30 sec,59 ℃ 30 sec,72 ℃ 90 sec)×32 cycle,72 ℃ 10 min
NPM-A3A-R	5'-CTGCCTTCCTTAGAGACTGAGGC-3'
NPM-A3B-F	5'-AGAGCGGGACTGGGACAAG-3'	94 ℃ 5 min, (94 ℃ 30 sec,59 ℃ 30 sec,72 ℃,2 min) × 32 cycle,72 ℃ 10 min
NPM-A3B-R	5'-TTAGAGACTGAGGCCCATCCTTC-3'
NPM-A3C-F	5'-ACAAGCGTATCTAAGAGGCCG-3'	94 ℃ 5 min, (94 ℃ 30 sec,59 ℃ 30 sec,72 ℃ 90 sec)×32 cycle,72 ℃ 10 min
NPM-A3C-R	5'-GGAGGCCCGTGCAGCATG-3'
NPM-A3D-F	5'-GCGTATCTAAGAGGCTGAACAT-3'	94 ℃ 5 min, (94 ℃ 30 sec,59 ℃ 30 sec,72 ℃,2 min) × 32 cycle,72 ℃ 10 min
NPM-A3D-R	5'-TTATGTTGGGGAGATGGGAAGAG-3'
NPM-A3F-F	5'-ACAAAGATCTTAGTCGGGACT-3'	94 ℃ 5 min, (94 ℃ 30 sec,59 ℃ 30 sec,72 ℃,2 min) × 32 cycle,72 ℃ 10 min
NPM-A3F-R	5'-AGGCTAGAGGAGACAGACCA-3'
NPM-A3G-F	5'-GTCAGGACTAGCCGGCAAAGGAT-3'	94 ℃ 5 min, (94 ℃ 30 sec,59 ℃ 30 sec,72 ℃,2 min) × 32 cycle,72 ℃ 10 min
NPM-A3G-R	5'-CTTCCTTAGAGACTGAGGCCCATC-3'
NPM-A3H-R	5'-GACCAGCAGGCTATGAGGCAA-3'	94 ℃ 5 min, (94 ℃ 30 sec,59 ℃ 30 sec,72 ℃ 90 sec)×32 cycle,72 ℃ 10 min
NPM-A3H-F	5'-CCAGAAGCACAGATCAGAAACACGAT-3'