Table 2.
Primer sequences and PCR conditions for molecular cloning of NPM A3s
| Primers | Primer DNA sequences | PCR conditions |
| NPM-A3A-CD-F | 5'-gcgaagcttgccaccATGGACGGCAGCCCAGCATCCAG-3' | 94 ℃ 5 min, (94 ℃ 30 sec,58 ℃ 30 sec,72 ℃ 90 sec) ×32 cycle,72 ℃ 10 min |
| NPM-A3A-CD-R | 5'-cctctagaggcTCACTTATCGTCGTCATCCTTGTAATCgccgccGTTTCCCTGATTCTGGAG AATGGCCC-3' | |
| NPM-A3B-CD-F | 5'-gcgaagcttgccaccATGAATCCACAGATCAGAAATCCGATGGAGC-3' | 94 ℃ 5 min, (94 ℃ 30 sec,58 ℃ 30 sec,72 ℃ 2 min) ×32 cycle,72 ℃ 10 min |
| NPM-A3B-CD-R | 5'-cctctagaggcTCACTTATCGTCGTCATCCTTGTAATCgccgccGTTTCCCTGATTCTGGAG AATGGCC-3' | |
| NPM-A3C-CD-F | 5'-gcaagcttgccaccATGAATCCACAGATCAGAAACCCGATGA-3' | 94 ℃ 5 min, (94 ℃ 30 sec,58 ℃ 30 sec,72 ℃ 90 sec) ×32 cycle,72 ℃ 10 min |
| NPM-A3C-CD-R | 5'-cctctagaggcTCACTTATCGTCGTCATCCTTGTAATCgccgccCTGAAGAATCTCCCGTAG GC-3' | |
| NPM-A3D-CD-F | 5'-gcgaagcttgccaccATGAATCCACAGATCAGAAATCCGATGGAGGG-3' | 94 ℃ 5 min, (94 ℃ 30 sec,58 ℃ 30 sec,72 ℃ 2 min) ×32 cycle,72 ℃ 10 min |
| NPM-A3D-CD-R | 5'-cctctagaggcTCACTTATCGTCGTCATCCTTGTAATCgccgccCTGAAGAATCTTCCATAG GCGTCTTTCCAGA-3' | |
| NPM-A3F-CD-F | 5'-gcgaagcttgccaccATGAAGCCTCAGTTCAGAAACACAGT-3' | 94 ℃ 5 min, (94 ℃ 30 sec,58 ℃ 30 sec,72 ℃ 2 min) ×32 cycle,72 ℃ 10 min |
| NPM-A3F-CD-R | 5'-CctctagaggcTCACTTATCGTCGTCATCCTTGTAATCgccgccCTCGAGAATCTCCTGCA GCTTG-3' | |
| NPM-A3G-CD-F | 5'-gcgaagcttgccaccATGAATCCTCAAATCAGAAACATGGTGGA-3' | 94 ℃ 5 min, (94 ℃ 30 sec,58 ℃ 30 sec,72 ℃ 2 min) ×32 cycle,72 ℃ 10 min |
| NPM-A3G-CD-R | 5'-cctctagaggcTCACTTATCGTCGTCATCCTTGTAATCgccgccGTTTCCCTGATTCTGGAG AATGGC-3' | |
| NPM-A3H-CD-F | 5'-gcgaagcttgccaccATGGCTCTGCTAACAGCCAAAACATTC-3' | 94 ℃ 5 min, (94 ℃ 30 sec,58 ℃ 30 sec,72 ℃ 90 sec) ×32 cycle,72 ℃ 10 min |
| NPM-A3H-CD-R | 5'-cctctagaggcTCACTTATCGTCGTCATCCTTGTAATCgccgccTCTTGAGTTGCGTATTGA CGATGAGGGG-3' |