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Fluorescence anisotropy measurements to determine the binding affinity (n = 3, Kd ± SD in nM) of the phosphorylated smAKAP-AKB domain peptide, tagged with 5-TAMRA (excitation at 535 nm and emission at 580 nm) with the full-length regulatory subunit dimers: PKA-RIα and PKA-RIβ. The smAKAP-wt, dAKAP2, and AKAP79 results were previously published [9].
| smAKAP-wt (nM) | smAKAP-P (nM) | dAKAP2 (nM) | AKAP79 (nM) | |
|---|---|---|---|---|
| RIα | 6.7 ± 0.7 | > 1000 | 50 | nd |
| RIβ | 6.9 ± 0.6 | > 1000 | – | nd |
