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. Author manuscript; available in PMC: 2016 Aug 10.
Published in final edited form as: J Am Coll Cardiol. 2008 Nov 11;52(20):1652–1660. doi: 10.1016/j.jacc.2008.06.051

Figure 1. Confirmation of hNIS-expression.

Figure 1

(a) Immunostaining confirmed the expression of hNIS (green) in rCDCs after lentiviral transduction (nuclei were counterstained by blue Hoechst dye). (b) Human NIS mRNA was detected by RT-PCR in rat hearts after transplantation of NIS+ rCDC (band at 353bps; white arrow I). White arrow for II indicates the lack of a specific band in a rat heart injected with the same number of NIS− cells. (c) Confirmation of hNIS activity in transduced cells. NIS transduction promoted in vitro 99mTc (pertechnetate) uptake by NIS+ rCDCs; this uptake was abolished by the specific NIS blocker sodium perchlorate (100μM), (two independent experiments, each condition tested in triplicate).