Skip to main content
. Author manuscript; available in PMC: 2016 Aug 10.
Published in final edited form as: Oncogene. 2014 Sep 22;34(28):3627–3639. doi: 10.1038/onc.2014.301

Figure 2. AMPK agonists stimulate AMPK-dependent signal transduction in MEFs.

Figure 2

Immunoblot for AMPKα (Thr172 and total), ACC (Ser79 and total), raptor (Ser792 and total), p70S6K (Thr389 and total), 4E-BP1 (different phosphorylation states marked by arrows) and actin on lysates from WT or DKO MEFs. Cells were incubated with metformin (5mM), phenformin (1.25mM), AICAR (1.25mM), 2DG (12.5mM), salicylate (2.5mM) or A-769662 (25μM) for 1h.