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. Author manuscript; available in PMC: 2016 Aug 10.
Published in final edited form as: Oncogene. 2014 Sep 22;34(28):3627–3639. doi: 10.1038/onc.2014.301

Figure 3. The majority of AMPK agonists inhibit proliferation in an AMPK-independent manner.

Figure 3

A) HEK293 cells were grown for 48h in the presence of metformin, phenformin, AICAR, 2DG, salicylate or A-769662 at the concentrations indicated. Adherent cell growth was determined by crystal violet staining. Data are expressed as mean ± SEM for triplicate cultures. B) Growth of WT or DKO MEFs was determined as in (A). C) WT and DKO MEFs were incubated for 24h with phenformin (0.63mM), AICAR (0.63mM), 2DG (6.25mM) or salicylate (2.5mM) before the percentage of cells in G1, S and G2/M phase were measured by propidium iodide staining. D) Growth of WT and DKO MEFs in the presence of A-769662 was determined as in (A). E) Cell cycle profiles of WT and DKO MEFs following treatment with A-769662 (25μM) was determined as in (C).