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. Author manuscript; available in PMC: 2016 Aug 10.
Published in final edited form as: Biochemistry. 2016 Aug 1;55(31):4286–4294. doi: 10.1021/acs.biochem.6b00124

Figure 3.

Figure 3

Recombinant OpaCEA proteins retain their ability to interact with CEACAM receptors. Opa proteins were recombinantly expressed, purified, and refolded into liposomes, and then incubated with NCCM1 (A) and NCCM3 (B). Samples were then centrifuged and the pellet (P) and supernatant (S) were assessed for the presence of Opa and NCCM. For Opa immunoblots, the two bands correspond to folded (lower band) and unfolded (upper band) protein. The intensity of NCCM pellet bands were quantified with ImageJ and plotted for each Opa – NCCM combination (C).