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. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: Integr Biol (Camb). 2016 Jun 24;8(8):813–820. doi: 10.1039/c6ib00077k

Fig. 1. Microfluidic perfusion and micropatterned collagen fiber surfaces.

Fig. 1

(A) An 8-channel microfluidic device (each channel: 250-μm wide × 60-μm high) for perfusion of whole blood over a patterned collagen feature. CTI (4 μg/ml)-treated whole blood was transferred to each reservoir within 5 min of phlebotomy and was immediately perfused over the collagen zone at venous or arterial wall shear rates (100 or 1000 s−1) controlled by a syringe pump connected at the outlet. Red dashed lines indicate location of patterned collagen (B, E). Collagen fibers were oriented perpendicularly to the flow channels (dark vertical lines are channel side walls). A 20-μm collagen zone was typically composed of 4 to 8 aligned collagen fibers (B, C). Single collagen fibers were confined within the 250-μm wide zone and no upstream or downstream collagen deposition was detected (E, F). Annexin V staining demonstrated TF liposomes (red) absorbed on fibers in the 20-μm collagen zone (D, cyan) or on a single collagen fiber (G, cyan). All scale bars: 50 μm in B-G.