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. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: Curr Biol. 2016 Jul 7;26(15):1965–1974. doi: 10.1016/j.cub.2016.05.076

Figure 2. The activity of SLC5A11 neurons is necessary and sufficient for hunger-driven behaviors.

Figure 2

(A) Representative traces of spontaneous activity and (B) quantification of firing frequency and bursting probability recorded from SLC5A11 neurons in the brains of fed flies carrying PSLC5A11-GAL4 alone (control) and fed flies carrying PSLC5A11-GAL4 and UAS-NaChBac (n = 12-13; ***P < 0.001). See also Figure S2. (C-E) Behavior responses of 5-hr (fed) or 22-hr (starved) food deprived flies carrying PSLC5A11-GAL4 and either UAS-NaChBac (n = 8-12; ***P < 0.001 vs. fed in the same genotype) (C), UAS-dTrpA1 (n = 6-8; ***P < 0.001 vs. 22 °C in the same genotype) (D), or UAS-Shits (n = 6-9; ***P < 0.001 vs. 22 °C in the same genotype) (E) in the two-choice assay. (F-H) Relative amounts of food consumed by 5-hr (fed) or 22-hr (starved) deprived flies bearing PSLC5A11-GAL4, and either UAS-NaChBac (F,G) or UAS-Kir2.1 (H) (n = 9-17; ***P < 0.001 vs. control). (I,J) Olfactory responses of 5-hr (fed) or 22-hr (starved) deprived flies bearing PSLC5A11-GAL4 or PR38H02-GAL4 with either UAS-Kir2.1 (I) or UAS-NaChBac (J) to 1% ACV versus water in a T-maze preference assay (n = 7-15; ***P < 0.001 vs. fed in the same genotype). Flies harboring either PSLC5A11-GAL4 or UAS-responder alone were used as controls. Error bars indicate SEM. See also Figure S4.