Skip to main content
. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: Curr Biol. 2016 Jul 14;26(15):2037–2043. doi: 10.1016/j.cub.2016.06.002

Figure 1. Expression of ATF5 rescues UPRmt activation in worms lacking ATFS-1.

Figure 1

(A) Schematic comparing the bZip transcription factors ATFS-1 and ATF5 including the mitochondrial targeting sequence (MTS), nuclear export sequence (NES) and the nuclear localization sequence (NLS).

(B) Schematic of the hsp-60pr::gfp reporter highlighting the three UPRmt elements in the promoter. The mutated element used in Figure 1D is marked with an asterisk (*).

(C) Photomicrographs of atfs-1(tm4525);hsp60pr::gfp worms expressing transgenic ATF5, ATF4 or ATFS-1 and raised on control, timm-23, spg-7, or ero-1(RNAi). Scale bar, 0.5 mm.

(D) Photomicrographs of wildtype and atfs-1(tm4525) worms expressing either hsp-60pr::gfp or hsp-60pr::gfp lacking a UPRmtE (*) (Figure 1B) raised on control or spg-7(RNAi). Worms in the right two panels express transgenic ATF5. Scale bar, 0.5 mm.

(E) Photomicrographs of control or transgenic ATF5 expressing hsp-4pr::gfp worms, raised on control or xbp-1(RNAi) incubated at 20°C or 30°C. Scale bar, 0.5 mm. See also Figure S1.