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. Author manuscript; available in PMC: 2016 Aug 10.
Published in final edited form as: Immunol Cell Biol. 2016 Feb 16;94(7):640–645. doi: 10.1038/icb.2016.19

Figure 2.

Figure 2

Absence of Id2 results in hyporesponsive iNKT cells. (a) CD4creID2f/f and CD4creID2+/+ mice were injected with 1 μg of αGalCer for 2 h. Total splenocytes were cultured for 4 h in the presence of monensin. iNKT cells were gated as in Figure 1b and analyzed for the expression of IL-4 and IFNγ. Representative fluorescence-activated cell sorting (FACS) plots and graph of iNKT cells from three mice. DN, double negative; DP, double positive for IFNγ and IL-4. (b) Analysis of iNKT cells from Id2YFP reporter mice 12 days after treatment with 1 μg of αGalCer or without treatment. Splenic iNKT cells were gated as in Figure 1b. Bar graphs indicate minimum to maximum and mean values of Id2 YFP geometric mean fluorescent intensity (gMFI) of three mice representative of two independent experiments. (c) Id2 YFP expression after iNKT cell activation. Mice were treated with 1 μg αGalCer or 40 μg LPS intraperitoneally. Splenic iNKT cells were gated as in Figure 1b and analyzed for Id2 YFP expression after 3 days (histogram), and 6 and 12 days (chart). Statistical significance was evaluated with unpaired Student’s t-test, where ***P>0.001.