Fig. 2.

FimL interacts directly with FimV and PilG by BACTH. β-galactosidase assays were performed on liquid-grown BTH101 E. coli transformed with the indicated plasmid pairs. Results are expressed as Miller units of β-galactosidase activity (A₄₂₀ min⁻¹ mL⁻¹ of cells measured at OD₆₀₀). Shown are Mean ± SEM (N = 3 biological replicates with three technical replicates).

A. ****P ≤ 0.0001 for T25-FimV/T18-FimL, T25-FimL/FimV-T18, FimL-T25/FimV-T18 and P ≤ 0.05 for T25-FimL/T18-FimV plasmid pairs relative to all controls.

B. ****P ≤ 0.0001 for all combinations of FimL and PilG relative to all controls.

C. **P ≤ 0.01 for PilH-T25/T18-FimL relative to all controls.

D. *P ≤ 0.05 for T25-FimV/T18-PilG relative to some controls (FimV-T25/T18-zip, T18-FimV/T25 and T25/T18).