A. Subsurface stab assays of the indicated strains. Shown are mean ± SEM (N = 5) of the twitching zone diameter measured at 24 h after inoculation. *** P ≤ 0.0001 compared to PAO1ΔfimL.
B. β-galactosidase activity (A420 min−1 mL−1 of cells measured at OD600) of the indicated strains expressing the lacp1-lacZ reporter gene. cAMP levels directly correlate with β-galactosidase activity from the lacp1 promoter. Shown are mean ± SEM (N = 5).
*** P ≤0.001 PAO1ΔfimL compared to PAO1ΔfimV.