Figure 1.

Preparation of purified recombinant human coatomer. (A) Schematic diagram showing the domain organization of each subunits of human coatomer. The available atomic structures with their PDB IDs and sequence coverage are also labeled. (B) Coomassie-blue stained SDS-PAGE of recombinant coatomer with all the subunits labeled. Asterisk indicates the N-terminal fragment of δ-COP, which is confirmed by mass spectrometry and Western blot. (C) Gel filtration of purified coatomer. The retention volume of the single peak on the column of Superose 6 (GE healthcare) is 11.4 mL. (D) Native PAGE of the purified coatomer. Arrow indicates the intact coatomer