Table 2.
Diversity of microorganisms per sampling site. FLA and Legionella isolated by culture and identified by DNA sequencing. Endosymbionts detected by FISH in isolates of FLA and identified by DNA sequencing
| FLA | Legionella | |||
|---|---|---|---|---|
| Taxa | No. of isolates | Endosymbionts | Species | No. of isolates |
| Cooling tower Hospital 1 | ||||
| Acanthamoeba genotype T4 | 3 | Paracaedibacter acanthamoebae | L. rubrilucens | 4 |
| Cochliopodium minus | 3 | Legionellales | ||
| Stenamoeba sp. | 2 | – | ||
| Thecamoeba sp. | 5 | – | ||
| Protostelium-like amoeba | 1 | – | ||
| Vahlkampfia avara | 1 | – | ||
| Cooling tower Hospital 2 | ||||
| Acanthamoeba genotype T4 | 5 | Rickettsiales | ||
| Vahlkampfiidaea | 1 | – | ||
| Naegleria clarki/pageib | 2 | – | ||
| Cooling tower Company | ||||
| Acanthamoeba genotype T4 | 1 | – | L. pneumophila SG 2-14 | 1 |
| Leptomyxa reticulata | 2 | – | ||
| Shower head | ||||
| – | – | – | L. pneumophila SG 2-14 | 1 |
| Total | 26 | 3 | 6 | |
aMixed culture of several genera grown at 30°
bThermophilic