Figure 4. Impaired CL-induced angiogenesis and beige transition in EC Vegfr2-specific eliminated mice.

(a) Microvessels (CD31⁺ red), adipocyte morphology (H&E; perilipin⁺ green), and UCP1 expression (UCP1⁺ red) of vehicle- and CL-treated gWAT in wt and Flk1f/f;Cdh5CreER^T2 mice. White arrows and arrowheads in upper three panels point to microvessels. Arrows in the lowest row of panels indicate UCP1-positive signals. Double-arrowed bars mark adipocyte diameter. n=5 for each wt and Flk1f/f;Cdh5CreER^T2 group. (b) Quantification of microvessel density in vehicle- and CL-treated wt and Flk1f/f;Cdh5CreER^T2 mice (n=10 random fields; n=5 mice for each group). (c) Quantification of average adipocyte size (>30 adipocytes/field; n=10 random fields; n=5 mice for each group). (d) Quantification of UCP1-positive signals (n=10 random fields; n=5 mice for each group). (e) qPCR quantification of mRNA expression levels Ucp1, Cidea, Cox8b, Pgc1α and Prdm16 in vehicle- and CL-treated total gWAT samples in wt and Flk1f/f;Cdh5CreER^T2 mice. (n=10 samples for each group). All scale bars, 50 μm. **P<0.01; ***P<0.001 by two-sided unpaired t-test. Data presented as mean±s.e.m.