Figure 2. Carbachol increases H₂S production in human urothelium and T24 cells.

(A,B) Carbachol (0.1, 1 and 10 μM) causes a significant increase in H₂S generation in human urothelium and in T24 cells, respectively (**p < 0.01 and ***p < 0.001 vs vehicle). (C,D) AOAA (1 mM), a CBS inhibitor, abolishes the increase in H₂S production induced by carbachol (1 μM) in human urothelium (***p < 0.001 vs vehicle, °°°p < 0.001 vs carbachol) and in T24 cells (***p < 0.001 vs vehicle, °°p < 0.01 vs carbachol). (E) Western blot analysis for CBS in T24 cells or T24ΔCBS cells, using β-actin as a protein loading control. (F) H₂S production in T24ΔCBS is not modified by carbachol (0.1, 1 and 10 μM) compared to vehicle. Data are calculated as nanomoles per milligram of protein per min and expressed as mean ± SE of four different experiments.