Figure 4. Muscarinic receptors involvement in carbachol induced increase in H₂S production.

(A) Semi quantitative RT-PCR for muscarinic receptors M1, M2, M3, M4, M5 in T24 cells and human urothelim respectively. (B) Telenzepine (10 nM) or 4DAMP (100 nM), M1 and M3 antagonists respectively, significantly reduces carbachol-induced increase in H₂S production in human urothelium (***p < 0.001 vs vehicle, °p < 0.05 and °°p < 0.01 vs carbachol). (C) The blockage of M1 receptor (telenzepine 10 nM) markedly reduces H₂S generation compared with carbachol in T24 cells (***p < 0.001 vs vehicle, °°°p < 0.001 vs carbachol). H₂S production data are calculated as nanomoles per milligram of protein per min and expressed as mean ± SE of four different experiments.